Technological advancement in medicine has helped come up with better diagnostic methods. The cardiac Elisa kits are the latest invention in this field. They are enzyme-dependent test devices that help in determining the presence or absence of heart diseases. These equipments are capable of discerning problems in hearts of virtually all animals.
This experiment works when the enzyme immunoassay binds with antibodies and substrate. When this occurs, color changes to indicate presence or absence of trouble. With these tools, it is possible to work with both antibodies and antigens. The amount of both of them can be determined by observing the color changes.
This test can also be used in detecting foreign bodies that exist in low concentrations. Heart problems can, therefore, be identified before they become chronic. The patient is advantaged; he will spend less money fighting a developing problem than he would have spent on a chronic one. This is because it is cheaper treating a disease while still in its early stages than when it has developed into a complex illness.
For proper working of these devices precision, sensitivity, accuracy and ability to work on a wide range of problems, and give many details are very important. Sensitivity helps in detecting any slight change in reaction when they are mixed with the samples. Accuracy, on the other hand, is required in ensuring that no errors are made in the experiments. The devices are also supposed to be specific to individual heart problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
This experiment works when the enzyme immunoassay binds with antibodies and substrate. When this occurs, color changes to indicate presence or absence of trouble. With these tools, it is possible to work with both antibodies and antigens. The amount of both of them can be determined by observing the color changes.
This test can also be used in detecting foreign bodies that exist in low concentrations. Heart problems can, therefore, be identified before they become chronic. The patient is advantaged; he will spend less money fighting a developing problem than he would have spent on a chronic one. This is because it is cheaper treating a disease while still in its early stages than when it has developed into a complex illness.
For proper working of these devices precision, sensitivity, accuracy and ability to work on a wide range of problems, and give many details are very important. Sensitivity helps in detecting any slight change in reaction when they are mixed with the samples. Accuracy, on the other hand, is required in ensuring that no errors are made in the experiments. The devices are also supposed to be specific to individual heart problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
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